基于SCoT分子标记的裸果木遗传多样性分析

为了揭示裸果木的遗传多样性和遗传结构,采用SCoT分子标记对新疆7个裸果木自然种群201个植株进行分析。结果表明12条引物共扩增出145个位点,在物种和种群水平上裸果木多态位点百分率分别为97.24%和76.45%,Nei's遗传多样性指数分别为0.263 8和0.213 7,Shannon's信息多样性指数分别为0.408 1和0.331 6,说明裸果木具有较高的遗传多样性,且物种水平遗传多样性略高于种群水平;遗传分化系数为0.183 9,说明其遗传变异主要来自种群内,种群间存在一定程度的分化;Mantel检验结果表明裸果木种群地理距离与遗传距离不存在显著相关性;运用主坐标分析（PCoA）和STRUCTURE分析对裸果木个体间亲缘关系聚类分析的结果存在一定差异,但总体上结果是一致的。裸果木的遗传多样性和遗传结构可能与进化历史、繁育系统、地理隔离和人为干扰有关。     

青海湖草原围栏对植物群落的影响兼论濒危动物普氏原羚的保护

青海省海晏县克图地区是我国特有动物普氏原羚 (Procapraprzewalskii)的主要分布地之一。我们于 2 0 0 1年 6月、7月和 8月在该地区开展了围栏内外植物群落的比较研究。围栏内无家畜放牧 ,主要草食野生动物为普氏原羚 ;围栏外为草食家畜放牧区。我们发现 ,自从 1999年建立草原围栏以后 ,围栏内外植物群落产生了明显差异 :(1)地上生物量。 6月份围栏外植物地上生物量显著低于围栏内 (df =39,p <0 .0 5 ) ;7、8月份围栏外植物地上生物量显著高于围栏内 (df=39,p <0 .0 5 )。 (2 )植物高度。 6月份围栏外的植物高度显著低于围栏内 (df =39,p <0 .0 5 ) ;7、8月份围栏外的植物高度与围栏内植物高度差异不显著 (df=39,p >0 .0 5 )。 (3)植物盖度。 6、7月份围栏外植物盖度显著低于围栏内 (df =19,p <0 .0 5 ) ;8月份围栏内外的植物盖度差异不显著 (df =19,p >0 .0 5 )。(4)牧草的比例。围栏外明显低于围栏内 (df =6 ,p <0 .0 5 ) ;围栏外非牧草的比例明显高于围栏内 (df =6 ,p <0 .0 5 )。 (5 )生物多样性。植物生长季节围栏外植物群落的生物多样性显著高于围栏内 (df =6 ,p <0 .0 5 )。在草原生态系统围栏内外植物群落研究结果比较的基础上 ,讨论了草食动物采食强度对草原生态系统植物群落影响和围栏与野生     

Equus przewalskii plasma protease inhibitor (Pi) system

A detailed biochemical characterization of four of the five previously described alleles of the plasma protease inhibitor (Pi) system of Equus przewalskii was performed using both one- and two-dimensional electrophoretic techniques. The proteins have been characterized in terms of isoelectric point, relative molecular mass, inhibitory activity to bovine trypsin and chymotrypsin, immunochemical cross-reactivity, terminal sialic acid content and enzyme:inhibitor complex formation and the oxidation sensitivity of this interaction. Using these functional criteria, only three loci (Spi 1, 2 and 3) were found to control the plasma Pi proteins of the E. przewalskii haplotypes. In contrast a fourth locus, Spi 4, was found in some E. caballus haplotypes. The significance of these results with respect to the complexity of the protein pattern exhibited by the equine Pi multigene family is discussed.     

Population data and a fourth allele for equine complement component 3 (C3)

The C3 polymorphism of equine serum or plasma revealed by agarose gel electrophoresis can be diagnosed with protein stain following acid protein fixation. In addition to the three alleles previously described (C31, C32, C33), a fourth allele (C34) was found. Population data for 25 domestic breeds and Equus przewalskii are presented.